cdkn2c antibody  (ABclonal Biotechnology)

Journal: Cancer medicine

Article Title: The expression and role of SUZ12 in lung adenocarcinoma.

doi: 10.1002/cam4.70190

Figure Lengend Snippet: FIGURE 8 The effect of SUZ12 on CKIs, p53, and Rb expression was tested by qRT-PCR and western blotting. sh-SUZ12 decreased p57 mRNA expression (A) and p18/p19/p-p53 protein expression (B) and (D), while increased p57/Rb/pRb protein expression (B) and (D), without significantly effected the p53 and Rb mRNA expression (C). *p < 0.05, **p < 0.001.

Article Snippet: The list of primary antibodies: SUZ12 (1 μg/mL, Abcam Cambridge, cat no: ab12073), CDK2 (1:1000; Proteintech, USA, cat. no. 10122- 1- AP), CDK3 (1:2000; Proteintech, USA, cat. no. 55103- 1- AP), CDK6 (1:2000; Proteintech, USA, cat. no. 14052- 1- AP), cyclin D1 (1:5000; Proteintech, USA, cat. no. 26939- 1- AP), cyclin E1 (1:1000; Proteintech, USA, cat. no. 11554- 1- AP), p18 (1:1000, BOSTER China, cat. no. M03299- 1), p19 (1:1000, BOSTER China, cat. no. MA1075), p53 (1:5000; Proteintech, USA, cat. no. 60283- 2- Ig), p- p53 (1:2000; Proteintech, USA, cat. no. 28961- 1- AP), p57 (1:1000, BOSTER China, cat. no. BM4129), Rb (1:1000, BOSTER China, cat. no. BM4500), pRb (1:1000, BOSTER China, cat. no. BM4338), Bcl- 2 (1:1000; Proteintech, USA, cat. no. 26593- 1- AP), Bax (1:2000; Proteintech, USA, cat. no. 50599- 2- lg), Ecadherin (1:5000; Proteintech, USA, cat. no. 20874- 1- AP), N- cadherin (1:3000; Proteintech, USA, cat. no. 22018- 1- AP), vimentin (1:4000; Proteintech, USA, cat. no. 10366- 1- AP), MMP1 (1:1000, BOSTER China, cat. no. A00733- 1), MMP2 (1:500, BOSTER China, cat. no. BM4075), MMP9 (1:1000, BOSTER China, cat. no. PB0709), MMP14 (1:1000, BOSTER China, cat. no. BM4119), TIMP1 (1:1000, Bioss China, cat. no. bs0415R), TIMP2 (1:1000, Bioss China, cat. no. bs- 10395R), TIMP3 (1:1000; Proteintech, USA, cat. no. 10858- 1- AP), ITGB1 (1:1000, BOSTER China, cat. no. BM4308), ITGB3 (1:1000, BOSTER China, cat. no. BA1670), ITGB5 (1:1000, BOSTER China, cat. no. A04201- 1), nm23 (1:1000, BOSTER China, cat. no. BA3787), PD- L1 (1:3000; Proteintech, USA, cat. no. 66248- 1- Ig), and βactin (1:5000; Proteintech, USA, cat. no. 66009- 1- Ig).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Scientific Reports

Article Title: Oxidative modification of miR-30c promotes cardiac fibroblast proliferation via CDKN2C mismatch

doi: 10.1038/s41598-024-63635-2

Figure Lengend Snippet: CDKN2C is the target of 4,5-oxo-miR-30c. ( A ) 4,5-oxo-miR-30c and homologies of its predicted target sites in the 3′UTR of CDKN2C mRNA. ( B ) Luciferase reporter assays for the 3′UTR of CDKN2C mRNA using synthesized 4,5-oxo-miR-30c, 4,5-mut-miR-30c, and miR-30c. Activity is reported relative to the nontarget control. *P < 0.05. ( C ) Representative Western blot (top) and densitometric analysis (bottom) of CDKN2C protein levels in miR-30c and 4,5-oxo-miR-30c NRCFs. *p < 0.05 compared to the control group.

Article Snippet: CDKN2C (catalog number, AF5356) was purchased from Affinity Bioscience (USA). β-Actin and HRP-conjugated secondary antibodies were purchased from Yesen Biotechnology (Shanghai, China).

Techniques: Luciferase, Synthesized, Activity Assay, Control, Western Blot

Journal: Scientific Reports

Article Title: Oxidative modification of miR-30c promotes cardiac fibroblast proliferation via CDKN2C mismatch

doi: 10.1038/s41598-024-63635-2

Figure Lengend Snippet: 4,5-oxo-miR-30c promotes cell proliferation by targeting CDKN2C in NRCFs. ( A ) Representative Western blot (top) and densitometric analysis (bottom) of CDKN2C protein levels in Ang II/PDGF-treated NRCFs. *p < 0.05 compared to the control group. Representative Western blot (top) and densitometric analysis (bottom) of Col1A protein levels in NRCFs treated with Ang II ( B ) PDGF ( C ) with or without CDKN2C overexpression. *p < 0.05 compared to the control group. ( D ) Cell proliferation (48 h) in NRCFs treated with 4,5-oxo-miR-30c with or without overexpression of CDKN2C or with or without CDKN2C siRNA. *p < 0.05 compared to the control group.

Article Snippet: CDKN2C (catalog number, AF5356) was purchased from Affinity Bioscience (USA). β-Actin and HRP-conjugated secondary antibodies were purchased from Yesen Biotechnology (Shanghai, China).

Techniques: Western Blot, Control, Over Expression

Journal: Frontiers in Molecular Biosciences

Article Title: Comprehensive analysis of senescence-related genes and immune infiltration in intervertebral disc degeneration: a meta-data approach utilizing bulk and single-cell RNA sequencing data

doi: 10.3389/fmolb.2023.1296782

Figure Lengend Snippet: Primer sequences used for qPCR.

Article Snippet: The sections were then incubated overnight at 4°C with primary antibodies: MAP4K4 (Rabbit, cat. no. 55247-1-AP, Proteintech, China), CXCL8 (Rabbit, cat. no. 17038-1-AP, Proteintech, China), TFDP2 (Rabbit, cat. no. 11500-1-AP, Proteintech, China), TNIK (Mouse, cat. no. 67948-1-Ig, Proteintech, China), CDKN2C (Rabbit, cat. no. AF0620, Affinity Biosciences, China).

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Comprehensive analysis of senescence-related genes and immune infiltration in intervertebral disc degeneration: a meta-data approach utilizing bulk and single-cell RNA sequencing data

doi: 10.3389/fmolb.2023.1296782

Figure Lengend Snippet: Probe sequences used for FISH.

Article Snippet: The sections were then incubated overnight at 4°C with primary antibodies: MAP4K4 (Rabbit, cat. no. 55247-1-AP, Proteintech, China), CXCL8 (Rabbit, cat. no. 17038-1-AP, Proteintech, China), TFDP2 (Rabbit, cat. no. 11500-1-AP, Proteintech, China), TNIK (Mouse, cat. no. 67948-1-Ig, Proteintech, China), CDKN2C (Rabbit, cat. no. AF0620, Affinity Biosciences, China).

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Comprehensive analysis of senescence-related genes and immune infiltration in intervertebral disc degeneration: a meta-data approach utilizing bulk and single-cell RNA sequencing data

doi: 10.3389/fmolb.2023.1296782

Figure Lengend Snippet: Transcription factors (TFs) and miRNAs prediction of key senescence-related differentially expressed genes (DEGs). (A) Box plots showing the expression levels of CABIN1, CDKN2B, CDKN2C, CXCL8, H1-5, MAP4K4, MAPK11, MDM2, MINK1, TFDP2, TINF2, and TNIK in the intervertebral disc degeneration (IDD) and control groups. Red represents the IDD group, blue represents the control group. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Interaction network diagram showing 25 associated miRNAs and 45 TFs for 12 key senescence-related DEGs.

Article Snippet: The sections were then incubated overnight at 4°C with primary antibodies: MAP4K4 (Rabbit, cat. no. 55247-1-AP, Proteintech, China), CXCL8 (Rabbit, cat. no. 17038-1-AP, Proteintech, China), TFDP2 (Rabbit, cat. no. 11500-1-AP, Proteintech, China), TNIK (Mouse, cat. no. 67948-1-Ig, Proteintech, China), CDKN2C (Rabbit, cat. no. AF0620, Affinity Biosciences, China).

Techniques: Expressing, Control

Journal: Frontiers in Molecular Biosciences

Article Title: Comprehensive analysis of senescence-related genes and immune infiltration in intervertebral disc degeneration: a meta-data approach utilizing bulk and single-cell RNA sequencing data

doi: 10.3389/fmolb.2023.1296782

Figure Lengend Snippet: Validating key senescence-related DEGs. (A) Representative images were obtained using magnetic resonance imaging, and NP tissues obtained from patients with intervertebral disc degeneration were stained with HE, Safranin O, and immunohistochemical staining for P16 and P21. (B) qPCR validate the expression levels of CABIN1, CDKN2B, CDKN2C, CXCL8, H1-5, MAP4K4, MAPK11, MDM2, MINK1, TFDP2, TINF2, and TNIK in IDD and control nucleus pulposus tissue. Red represents the IDD group, blue represents the control group. * p < 0.05, ** p < 0.01. Representative images of CDKN2C, MAP4K4, TFDP2, TNIK, and CXCL8 expression in NP tissue detected by FISH (C) and Immunohistochemistry (D) .

Article Snippet: The sections were then incubated overnight at 4°C with primary antibodies: MAP4K4 (Rabbit, cat. no. 55247-1-AP, Proteintech, China), CXCL8 (Rabbit, cat. no. 17038-1-AP, Proteintech, China), TFDP2 (Rabbit, cat. no. 11500-1-AP, Proteintech, China), TNIK (Mouse, cat. no. 67948-1-Ig, Proteintech, China), CDKN2C (Rabbit, cat. no. AF0620, Affinity Biosciences, China).

Techniques: Magnetic Resonance Imaging, Staining, Immunohistochemical staining, Expressing, Control, Immunohistochemistry

Journal: Frontiers in Molecular Biosciences

Article Title: Comprehensive analysis of senescence-related genes and immune infiltration in intervertebral disc degeneration: a meta-data approach utilizing bulk and single-cell RNA sequencing data

doi: 10.3389/fmolb.2023.1296782

Figure Lengend Snippet: Receiver operating characteristic (ROC) curve. (A) ROC curve of CABIN1. (B) ROC curves of CDKN2B. (C) ROC curves of CDKN2C. (D) ROC curve of CXCL8. (E) ROC curves of H1-5. (F) ROC curve of MAP4K4. (G) ROC curve of MAPK11. (H) ROC curves of MDM2. (I) ROC curve of MINK1. (J) ROC curve of TFDP2. (K) ROC curve of TINF2. (L) ROC curves of TNIK.

Article Snippet: The sections were then incubated overnight at 4°C with primary antibodies: MAP4K4 (Rabbit, cat. no. 55247-1-AP, Proteintech, China), CXCL8 (Rabbit, cat. no. 17038-1-AP, Proteintech, China), TFDP2 (Rabbit, cat. no. 11500-1-AP, Proteintech, China), TNIK (Mouse, cat. no. 67948-1-Ig, Proteintech, China), CDKN2C (Rabbit, cat. no. AF0620, Affinity Biosciences, China).

Techniques: